Cloning and Characterization of bla VIM , a New Integron-Borne Metallo-β-Lactamase Gene from a Pseudomonas aeruginosa Clinical Isolate

Cloning and Characterization of blaVIM, a New Integron-Borne Metallo-b-Lactamase Gene from a Pseudomonas aeruginosa Clinical Isolate

LAURA LAURETTI, MARIA LETIZIA RICCIO, ANNARITA MAZZARIOL, GIUSEPPE CORNAGLIA, GIANFRANCO AMICOSANTE, ROBERTA FONTANA, AND GIAN MARIA ROSSOLINI* Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100-Siena, Istituto di Microbiologia, Università di Verona, 37134-Verona, and Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell’Aquila, 6710...

Cloning and characterization of blaVIM, a new integron-borne metallo-beta-lactamase gene from a Pseudomonas aeruginosa clinical isolate.

Production of a metallo-beta-lactamase activity was detected in a carbapenem-resistant Pseudomonas aeruginosa clinical isolate (isolate VR-143/97) from an Italian inpatient at the Verona University Hospital (northern Italy). The metallo-beta-lactamase determinant was isolated from a genomic library of VR-143/97, constructed in an Escherichia coli plasmid vector, by screening for clones with red...

A Novel Integron Gene Cassette Harboring VIM-38 Metallo-β-lactamase in a Clinical Pseudomonas aeruginosa Isolate

Fatih Şaban Beriş, Ph.D., Esma Akyildiz, M.S., Azer Özad Düzgün, Ph.D., Umut Safiye Say Coşkun, M.D., Cemal Sandalli, Ph.D., and Ayşegül Çopur Çiçek, M.D. Department of Biology, Faculty of Arts & Sciences, Recep Tayyip Erdoğan University, Rize; Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, Gümüşhane; Department of Medical Microbiol...

Characterization of VIM-2, a carbapenem-hydrolyzing metallo-beta-lactamase and its plasmid- and integron-borne gene from a Pseudomonas aeruginosa clinical isolate in France.

Pseudomonas aeruginosa COL-1 was identified in a blood culture of a 39-year-old-woman treated with imipenem in Marseilles, France, in 1996. This strain was resistant to beta-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible to the monobactam aztreonam. The carbapenem-hydrolyzing beta-lactamase gene of P. a...

Characterization of VIM-2, a Carbapenem-Hydrolyzing Metallo- b-Lactamase and Its Plasmid- and Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in France